Bone

Original Editors Candace Goh

Top Contributors - Candace Goh, Lucinda hampton, Shaimaa Eldib, Kim Jackson and George Prudden

Introduction

The adult human skeleton is composed of 206 bones.  At birth, there are approximately 270 bones, with the final adult count decreasing as a portion of these bones fuse during phases of skeletal growth and maturation. 

  • Bone is a metabolically active connective tissue that provides structural support, facilitates movement, and protects vital organs.
  • It plays an important role in regulating mineral and acid-base balance homeostasis. It also provides the environment for hematopoiesis (blood cells production) within the bone marrow. 
  • Bone is composed of an extracellular matrix and bone cells (osteocytes)[1]. Under the right conditions, bone tissue undergoes a process of mineralization, formed by collagen matrix and hardened by deposited calcium. Bone tissue (osseous tissue) differs greatly from other tissues in the body. Bone is hard and many of its functions depend on that characteristic hardness.[2]The skeleton is composed of around 80% cortical bone and 20% trabecular bone.[3]
  • Much of the cellular activity in a bone consists of removal and replacement at the same site, a process called remodelling. The remodelling process occurs throughout life and becomes dominant by the time that bone reaches its peak mass (typically by the early 20s). Remodelling continues throughout life so that most of the adult skeleton is replaced about every 10 years.[4]
[5]

Structure

The four general categories of bones are:

  • long bones : include the clavicles, humeri, radii, ulnae, metacarpals, femurs, tibiae, fibulae, metatarsals, and phalanges
  • short bones: include the carpal and tarsal bones, patellae, and sesamoid bones.
  • flat bones: include the skull, mandible, scapulae, sternum, and ribs.
  • irregular bones. include the vertebrae, sacrum, coccyx, and hyoid bone.

Flat bones form by membranous bone formation, whereas long bones are formed by a combination of endochondral and membranous bone formation.[6]

Gross anatomy:

Bone 1.jpg

A long bone has two parts: the diaphysis and the epiphysis. The diaphysis is the tubular shaft that runs between the proximal and distal ends of the bone. The hollow region in the diaphysis is called the medullary cavity, which is filled with yellow marrow. The walls of the diaphysis are composed of dense and hard compact bone.[7]

Individual bone structure

Architecturally, bone categorizes into two subtypes: cortical and trabecular bones.

  1. Cortical Bone: consists of about 80% of the total bone in the body and is much stronger than trabecular bone. It is very resistant to bending, torsion, and compression and is much more dense with a minimal role in metabolism. It is seen mostly in the shaft of long bones like the femur and the tibia as well as in the outer shell of trabecular bone.
  2. Trabecular Bone (spongy bone): consists of only 20% of the total bone but has ten times the surface/volume ratio of cortical bone. It responds eight times faster to changes in load making it far more dynamic. It occurs in areas that more subject to compression such as the vertebral body, pelvis, and the metaphyses.[8]

Cellular structure

As a specialized connective tissue, bone is comprised of mainly type 1 collagen fibers and inorganic salts.

Type 1 collagen is a non-calcified matrix (osteoid) designed to resist the tension experienced by the weight-bearing functions of bone. It forms from osteoblasts, the main cellular component of bone (osteoid, a gelatinous substance made up of collagen, a fibrous protein, and mucopolysaccharide, an organic glue. Soon after the osteoid is laid down, inorganic salts are deposited in it to form the hardened material recognized as mineralized bone).

Bone Cells 

Bone cells make up about 10% of total bone volume. There are four types of cell.

Osteoblasts produce matrix, build up bone tissue. They function in the production, transport, and arrangement of the osteoid matrix. Furthermore, they initiate and regulate matrix mineralization and control the neighboring bone cells activities.  

Once the osteoid is mineralized, the precursor cells get surrounded by organic intracellular substances called lacunae to become fully developed and matured into osteocytes. The mineral content is chiefly hydroxyapatite crystals composed of mainly calcium and phosphate ions but can occasionally have sodium and carbonite as well.

Osteocytes maintain bone tissue by mineralising osteoporosis. They have a widespread distribution. Their cell processes are fundamental to allows osseous tissue to be responsive to the mechanical and metabolic organism constraints.

Bone 2.jpg
Osteoclasts are the large, multinucleated cells on the surface of bones in pits called Howship lacunae. They are responsible for removing calcium from bone by secreting proteolytic enzymes and hydrogen ions to dissolve the calcium hydroxyapatite crystals. On average, osteoclasts have 12 nuclei, though the number may range from 2 to as many as 100. In their cytoplasm, interconnecting actin filaments produce a clear area between the cell membrane and the nuclei

Osteons represents a single functional unit of bone tissue. It is arranged with concentric lamellae of collagen fiber orientations around a central canal consisting of osteocyte’s arterial, venous, and nerve supply, is known as the Haversian canal. This system also consists of the canaliculi and Volkmann canal which allow for communication between neighboring osteocytes and communication between neighboring osteons, respectively.[8]

Molecular structure

Approximately 20% of in vivo bone is water. Of the dry bone mass, 60-70% of is bone mineral in the form of small crystals and the rest is collagen. The composition of the mineral component is hydroxyapatite Ca10(PO4)6(OH)2 and collagen is the main fibrous protein of the human body.

Functions

Mechanical

  • Bones provide a frame for other soft tissues of the musculoskeletal system to attach to such as muscles, tendons, and ligaments. These allow for support as well as the movement by contracting and relaxing of the muscles which then, in turn, result in flexion, extension, abduction, adduction, and other forms of movement.
  • Form a mechanical barrier to different structures within the human body. For example, the rib cage and the skull help shield our vital organs, the heart/lungs, and the brain, respectively, from trauma.[8]

Synthetic

The marrow is found in the trabecular portions of bones and is responsible for hematopoiesis, or the production of red blood cells, white blood cells, and platelets.[8]

Metabolic;[6]

  • The bone matrix can store several minerals, chiefly calcium and phosphorus as well as iron in the form of ferritin.
  • Chondroitin sulfate, a carbohydrate moiety, is also a commonly found element in the matrices.
  • Specific growth factors, including insulin-like growth factor or IGF-1, are housed in bone and then released periodically.
  • pH balance is also regulated as bones may alter the composition of alkaline salts in the serum to maintain the optimal pH level.
  • Osteocytes can engulf toxic molecules and heavy metals from the serum as a means of detoxification
  • Fat storage[8]

Remodelling

  1. This is a physiological process in which old or damaged bone is removed by osteoclasts and then replaced by new bone formed by osteoblasts.
  2. There is a tight coupling of bone formation to bone resorption to ensure no net change in bone mass or quality after each remodeling.
  3. It requires coordinated action of the four types of bone cells. The process involves four major distinct but overlapping phases:
  • Phase 1: initiation/activation of bone remodeling at a specific site. The osteoclast precursors are recruited to bone remodeling compartments (BRC).
  • Phase 2: bone resorption and concurrent recruitment of osteoprogenitors. Bone resorption represents the predominant event, but the recruitment of mesenchymal stem cells (MSCs) and/or osteoprogenitors into the BRC is also initiated.
  • Phase 3: osteoblast differentiation and function (osteoid synthesis). Excavated bone is replaced with osteoid produced by osteoblasts.
  • Phase 4: mineralization of osteoid and completion of bone remodeling. The osteoid is mineralized, and the bone remodeling cycle is concluded[1]


Clinical Significance

Bone tissue is susceptible to a myriad of pathologies that may range from etiologies of embryological, metabolic, autoimmune, neoplastic, or idiopathic origins. These include, but are not limited to, the conditions discussed below.

  • Achondroplasia is a genetic disorder commonly associated as a cause of dwarfism. Individuals affected may present with short extremities due to decreased development of endochondral bone.
  • Paget disease of the bone is characterized to be an imbalance amongst the activities of osteoblasts and osteoclasts. Of unknown etiology, the condition only affects localized portions of the skeletal tissue, generally involving one or more neighboring bones rather than the diffuse skeletal system.
  • If untreated, Paget disease of the bone can act as a risk factor for osteosarcoma, a malignant proliferation of osteoblasts.
  • Skeletal neoplasm begins in the metaphysis of long bones with patients complaining of bone pain with swelling or as a pathologic fracture (a break in the bone caused by weakness of the bone through disease rather than trauma). Much more common in teenagers than in the elderly.[8]
  • Bone fractures
  • Osteoporosis. This is a common disorder of bone remodeling which is characterized by low bone mass and structural deterioration of bone. It causes bone fragility and increased vulnerability to fractures. [1]
  • Osteoarthritis
  • Osteomalacia
  • Rickets
  • Epiphyseal plate disorders

References

  1. 1.0 1.1 1.2 El Sayed SA, Nezwek TA, Varacallo M. Physiology, Bone. InStatPearls [Internet] 2019 Jul 29. StatPearls Publishing. Available from:https://www.ncbi.nlm.nih.gov/books/NBK441968/ (last accessed 10.2.2020)
  2. Opentextbc.ca. (2018). 6.3 Bone Structure – Anatomy and Physiology. [online] Available at: https://opentextbc.ca/anatomyandphysiology/chapter/6-3-bone-structure/
  3. Singh S, Bray TJ, Hall-Craggs MA. Quantifying bone structure, micro-architecture, and pathophysiology with MRI. Clinical radiology. 2018 Mar 1;73(3):221-30.
  4. Office of the Surgeon General (US. The Basics of Bone in Health and Disease. InBone Health and Osteoporosis: A Report of the Surgeon General 2004. Office of the Surgeon General (US). Available from:https://www.ncbi.nlm.nih.gov/books/NBK45504/ (last accessed 10.2.2020)
  5. Types of bones in the human skeleton - Human Anatomy - Kenhub. [Last accessed - 13 July 2020}
  6. 6.0 6.1 Clarke B. Normal bone anatomy and physiology. Clinical journal of the American Society of Nephrology. 2008 Nov 1;3(Supplement 3):S131-9.
  7. Hall JE. Guyton and Hall textbook of medical physiology e-Book. Elsevier Health Sciences; 2015 May 31.
  8. 8.0 8.1 8.2 8.3 8.4 8.5 Baig MA, Bacha D. Histology, Bone. InStatPearls [Internet] 2019 May 5. StatPearls Publishing. Available from:https://www.ncbi.nlm.nih.gov/books/NBK541132/ (last accessed 10.2.2020)