Arterial Blood Gases

Original Editor - Scott Buxton

Top Contributors - Adam Vallely Farrell, Kim Jackson, Scott Buxton, Rachael Lowe and Laura Ritchie  

Arterial Blood Gases

Blood Gas Analyser
Arterial blood gases (ABG's) is a blood test which is used to give an indication of ventilation, gas exchange and acid-base status and is taken from an arterial blood supply[1]. It should be noted that it is not to be confused with venous blood gases which are used when arterial supply is not available or unreliable due to disease. The line in is usually inserted in the radial artery located at the wrist but is also sometimes used in the femoral artery in the groin[1]. This is important to consider when moving and handling a patient as it is easy to catch a line on an object or clothing and care needs to be taken.

It can be argued that one of the most important clinical uses of ABG analysis is to assess if a patient is in Type 1 (Hypoxaemia) or Type 2 respiratory failure and it is important that as physiotherapists we able to quickly and correctly interpret this.  

ABGs give us information about the activity in both the respiratory system and the 'metabolic' system. If one system is disturbed, the other will try to restore the balance or compensate. Both systems work together in an attempt to keep pH in the normal range. They are commonly used to; identify acid/base disorders, identify gas exchange problems, monitor the effects of oxygen therapy[2].

ABG Definitions

  • PH: The measure of hydrogen ions in the blood
  • PaC02: Partial pressure of Carbon Dioxide in the blood, the acidic element of the balance
    • Acidic component
    • Indicator of respiratory function
    • Changes rapidly to compensate
  • Pa02: Partial pressure of oxygen in the blood.
  • HCO3-: Bicarbonate ion concentration in the blood, the basic element of the balance
    • Basic/Alkaline component
    • Indicator of metabolic function
    • Compensation is slower
  • BE: Base Excess = quantity of strong acid or base that is required to restore pH to normal[3]

Normative Values[2] [4]

Arterial Blood Analysis Reference Ranges Venous Blood analysis Reference Ranges
pH 7.35 - 7.45 pH 7.31  - 7.41

10.7 - 13.3 kPa


5.0 - 5.6 kPa

PaCO2 4.7 - 6.0 kPa PCO2 5.6 - 6.7 kPa



22 - 26 mmol/L
Base Excess -2 to +2

Acid-Base Balance

  • pH
    • Reflects acid-base balance and responds to metabolic and respiratory changes. Body cells and chemical reactions are acutely sensitive to the pH of their environment.
      • Lower pH = Acidic
      • Higher pH= Alkaline
  • Regulation
    • Acid-base balance is disturbed if; CO2 removal by the lungs is abnormal, production of acid from tissues is abnormal, and removal of acid is abnormal.[2]
  • Deviation from normal pH is resisted by 3 mechanisms;
  1. The Buffering system
    • Acts like a chemical sponge and neutralises acids and bases. It is an acid-base homeostatic mechanism involving the balance of carbonic acid (H2CO3), bicarbonate ion (HCO3), and carbon dioxide (CO2) that attempts to maintain the bloods pH. It also plays a role in the duodenum among other tissues, to support proper metabolic function. [5]
  2. The Lungs
    • The respiratory component that reacts if the buffering system is not enough and the lungs help my regulating CO2.
  3. The Kidneys
    • The Metabolic component and is the last mechanism to work and begins to eliminate acid.

Respiratory Acidosis

Respiratory acidosis is caused by inadequate alveolar ventilation leading to the retention of carbon dioxide and an increase in free hydrogen ions. It occurs when decreased ventilation (hypoventilation) increases the concentration of carbon dioxide in the blood and results in a decrease in the blood's pH. [6]

  • Decreased pH
  • Increased PaCO2
  • Causes
    • Hypoventilation
    • Acute Lunge Injury
      • Upper airway obstruction
      • Lower airway obstruction
      • Impaired alveolar filling
    • Chronic Lung Disease
    • Neuromuscular disorders
    • Obesity
    • CNS Depression
  • Symptoms
    • Headache
    • Anxiety
    • Blurred vision
    • Restlessness
    • Drowsiness
    • Tremors
    • Delirium
    • Coma[2]

Metabolic Acidosis

Metabolic acidosis involves excess fixed acid production, i.e. lactate or loss of HCO3.It occurs when the body produces excess quantities of acid or the kidneys are not removing sufficient acid from the body. If left untreated, metabolic acidosis can lead to acidemia. Acidemia occurs when the bloods pH is low (<7.35) due to an increased production of hydrogen ions by the body or its inability to form bicarbonate (HCO3) in the kidneys. 

  • Decreased pH
  • Decreased HCO3
  • Causes
      • Renal disease
      • Liver disease
      • Lactic acidosis
      • Prolonged lack of oxygen
      • Shock
      • Posioning
      • Medications
      • Dehydration
      • Diarrhoea
  • Symptoms
    • Rapid breathing
    • Confusion
    • Lethargy
    • Cold, clammy skin
    • Tachycardia and arrhythmia[2]

Respiratory Alkalosis

Respiratory alkalosis is caused by over excretion of carbon dioxide (hyperventilation) resulting in more CO2 than normal being exhaled. Thus, leading to a reduction in free hydrogen ions and an alkalotic state.[7]

  • Increased pH
  • Decreased PaCO2
  • Causes
    • Hyperventilation
    • Acute asthma
    • CNS disturbance
    • High altitude
    • Pneumonia
    • Drugs
    • Fever
    • Sepsis
  • Symptoms
    • Diziness
    • Peripheral paraesthesia
    • Confusion
    • Dry mouth
    • Bloating[7]

Metabolic Alkalosis

Metabolic alkalosis occurs as a result of decreased hydrogen ion concentration which leads to increased bicarbonate, or alternatively a direct result of increased bicarbonate concentrations.[8]  

  • Increased pH
  • Increased HCO3
  • Causes
    • Excess alkali administration
    • Hyperkalaemia
    • IV penicillin
    • Re-feeding syndrome
    • Massive blood transfusion
    • Diuretic therapy
    • Vomitting
  • Symptoms
    • Weakness
    • Myalgia
    • Polyuria
    • Cardiac arrhythmias
    • Hypoventilation[2][8]

Respiratory Failure

Respiratory failure is characterized by a reduction in function of the lungs due to lung disease or a skeletal or neuromuscular disorder.[9] It occurs when gas exchange at the lungs is significantly impaired to cause a drop in blood levels of oxygen (hypoxemia) occurring with or without an increase in carbon dioxide levels(hypercapnia). It's usually defined in terms of the gas tensions in the arterial blood, respiratory rate and evidence of increased work of breathing[10]. Respiratory failure can be acute, chronic or acute on chronic.[11] It's a major cause of mortality and morbidity and mortality rates increase with age and presence of co-morbidities. Respiratory failure is not a disease but a consequence of the problems that interfere with the ability to breathe.[9]

It is the inability to perform adequately the fundamental functions of respiration:

  1. To deliver oxygen to the blood
  2. To eliminate carbon dioxide from it

Interpretation of ABGs

  1. Look at the pH
    • Increased = Alkalosis
    • Decreased = Acidosis
  2. Look at the PaCO2
    1. Increased = Respiratory Acidosis
    2. Decreased = Respiratory Alkalosis
  3. Look at the HCO3
    • Increased = Metabolic Alkalosis
    • Decreased = Metabolic Acidosis
  4. Look at the O2


The bodies pH is closely controlled and this is done through various mechanisms to maintain it at a constant value. It is important to note that the body will never overcompensate as the drivers for compensation cease as the pH returns to normal. In practice, compensation for an acidosis will not cause an alkalosis or visa versa.

If pH is NORMAL despite an abnormal PCO2 and HCO3 it must be compensated.

  1. Look at the pH - Which side of 7.4 is it?
  2. Look for the cause
    • PCO2 goes in the opposite direction to pH
    • HCO3 travels in the same direction as pH
  3. The other value is the compensator

Finding compensated, partially compensated, or uncompensated ABG problems:

  • When PaCO2 is high, but pH is normal instead of being acidic, and if HCO3 levels are also increased, then it means that the compensatory mechanism has retained more HCO3 to maintain the pH.
  • When PaCO2 and HCO3 values are high but pH is acidic, then it indicates partial compensation. It means that the compensatory mechanism tried but failed to bring the pH to normal.
  • If pH is abnormal and if the value of either PaCO2 or HCO3 is abnormal, it indicates that the system is uncompensated. This is probably because of either respiratory or metabolic acidosis.[13]

 Helpful guidelines[14]
1.A 1mmHg change in PaCO2 above or below 40 mmHg results in 0.008 unit change in pH in the opposite direction.
2.The PaCO2 will decrease by about 1 mmHg for every 1 mEq/L reduction in [HCO3-] below 24 mEq/L
3.A change in [HCO3-] of 10 mEq/L will result in a change in pH of approximately 0.15 pH units in the same direction.

The results should always be read and compared in reference to the patients previous ABG (if available) as you will then be able to assess a trend and make a more accurate assessment on whether you should treat or if your treatment has be successful or not.


Useful Resources

ABG Calculator

ABG's at altitude

Acid-Base Questions

ABG Interpretation Quiz

Associated Topics


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  2. 2.0 2.1 2.2 2.3 2.4 2.5 Woodrow P. Arterial blood gas analysis. Nursing Standard (through 2013). 2004 Feb 4;18(21):45.
  3. Buck RP, Rondinini S, Covington AK, Baucke FG, Brett CM, Camoes MF, Milton MJ, Mussini T, Naumann R, Pratt KW, Spitzer P. Measurement of pH. Definition, standards, and procedures (IUPAC Recommendations 2002). Pure and applied chemistry. 2002 Jan 1;74(11):2169-200.
  4. Kenyon K, Kenyon J. The Physiotherapist's Pocketbook. Essential Facts at your Fingertips. 2nd ed. London: Churchill Livingstone, Elsevier. 2009.
  5. Krieg, Brian J.; Taghavi, Seyed Mohammad; Amidon, Gordon L.; Amidon, Gregory E. (2014-11-01). "In Vivo Predictive Dissolution: Transport Analysis of the CO2, Bicarbonate In Vivo Buffer System"Journal of Pharmaceutical Sciences103 (11): 3473–3490. 
  6. Yee AH, Rabinstein AA (February 2010). "Neurologic presentations of acid-base imbalance, electrolyte abnormalities, and endocrine emergencies". Neurol Clin28 (1): 1–16
  7. 7.0 7.1 "Respiratory alkalosis: MedlinePlus Medical Encyclopedia"
  8. 8.0 8.1 Singh AK. Metabolic alkalosis. In: Mushlin SB, Greene HL II, eds. Decision Making in Medicine: An Algorithmic Approach. 3rd ed. Philadelphia, PA: Mosby Elsevier; 2010. 374-5.
  9. 9.0 9.1 Respiratory Failure. (2017, October 5). Physiopedia, . Retrieved 10:12, March 27, 2018 from
  10. Tulaimat A, Patel A, Wisniewski M, Gueret R. The validity and reliability of the clinical assessment of increased work of breathing in acutely ill patients. August 2016. Journal of crit care 34:111-5
  11. Pandor A, Thokala P, Goodacre S, Poku E, Stevens JW, Ren S, Cantrell A, Perkins GD, Ward M, Penn-Ashman J. Pre-hospital invasive ventilation for acute respiratory failure: a systematic review and cost effectiveness evaluation. Health Technol Assess 2015 June 19(42): 1-102.
  14. Stoeltin RK, Miller RD. Basics of Anesthesia, 5th ed. Philadelphia: Churchill Livingstone, Elsevier. 2007.
  15. Arterial Blood Gas (ABG) Tic-Tac-Toe Examples. Available from:[last accessed 27/03/18]
  16. 6 Easy Steps to ABG Analysis. Available from:[last accessed 27/03/18]
  17. ABGs (Arterial Blood Gas). Available from:[last accessed 27/03/18]