Blood Tests: Difference between revisions
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'''Original Editor '''- [[User:John mitchell|John Mitchell ]] | '''Original Editor '''- [[User:John mitchell|John Mitchell ]] | ||
'''Top Contributors''' - {{Special:Contributors/{{FULLPAGENAME}}}} | '''Top Contributors''' - {{Special:Contributors/{{FULLPAGENAME}}}} | ||
</div> | </div> | ||
== An Introduction To Pathology == | == An Introduction To Pathology == | ||
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== Chemical Pathology == | == Chemical Pathology == | ||
NB reference ranges apply to '''adults only '''and are laboratory specific and '''not applicable to every lab result ''' | NB reference ranges apply to '''adults only '''and are laboratory-specific and '''not applicable to every lab result ''' | ||
=== Water and sodium === | === Water and sodium === | ||
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↓K- '''''Hypokalemia''''' (alkalosis, RF, D&V,diuretics) – Weakness, ileus, hypotonia, confusion, depression, arrhythmias, alkalosis.<br>↑K- '''''Hyperkalemia''' (catabolism, acidosis, RF) – Cardiac arrest with VF.'' | ↓K- '''''Hypokalemia''''' (alkalosis, RF, D&V,diuretics) – Weakness, ileus, hypotonia, confusion, depression, arrhythmias, alkalosis.<br>↑K- '''''Hyperkalemia''' (catabolism, acidosis, RF) – Cardiac arrest with VF.'' | ||
=== Renal Function Tests - Urea & creatinine | === Renal Function Tests - Urea & creatinine === | ||
U: 2.5-6.5 mmol/l Cr: 60-120μmol/l<br> | U: 2.5-6.5 mmol/l Cr: 60-120μmol/l<br> | ||
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Both should rise together in renal failure. Creat is the more accurate measurement, urea is affected more by diet and dehydration.<br>Creatinine Clearance Crcl: 100-120mls/min<br>Measures glomerular filtration rate and permeability | Both should rise together in renal failure. Creat is the more accurate measurement, urea is affected more by diet and dehydration.<br>Creatinine Clearance Crcl: 100-120mls/min<br>Measures glomerular filtration rate and permeability | ||
=== Enzymes | === Enzymes === | ||
*Non specific | *Non-specific | ||
*Have a specific time window of elevation | *Have a specific time window of elevation | ||
*Temperature specific | *Temperature specific | ||
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Cholestasis, cirrhosis, hepatitis, liver tumour. | Cholestasis, cirrhosis, hepatitis, liver tumour. | ||
==== Alanine transaminase | ==== Alanine transaminase ==== | ||
Alt/GPT: <40 IU/l<br> | Alt/GPT: <40 IU/l<br> | ||
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=== Thyroid diseases<br> === | === Thyroid diseases<br> === | ||
'''Hyperthyroidism''' (Graves disease, multinodular | '''Hyperthyroidism''' (Graves disease, multinodular goiter, adenoma) – weight loss, sweating, palpitation, angina, tremor, diarrhea, muscle weakness, goiter, eyelid retraction. | ||
'''Hypothyroidism''' (Hashimotos disease, post surgery, congenital, secondary to pituitary/hypothalamic disease)<br>- lethargy, cold intolerance, dry coarse skin & hair, hoarseness, wt gain, slow reflexes & muscle relaxation. Others including: anaemia, psychosis, constipation bradycardia, CTS, infertility.<br> | '''Hypothyroidism''' (Hashimotos disease, post surgery, congenital, secondary to pituitary/hypothalamic disease)<br>- lethargy, cold intolerance, dry coarse skin & hair, hoarseness, wt gain, slow reflexes & muscle relaxation. Others including: anaemia, psychosis, constipation bradycardia, CTS, infertility.<br> | ||
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alb: 36-50 g/l<br> | alb: 36-50 g/l<br> | ||
* | *Maintains oncotic pressure (keeps fluid in vessels) | ||
*Transports small drugs, calcium & hormones | *Transports small drugs, calcium & hormones | ||
*↓ (Many causes incl. malnutrition, liver disease, overhydration, incr. cap. Permeability, protein losing states, burns, haemorrhage, general catabolism-sepsis, fever, malignancy, trauma) | *↓ (Many causes incl. malnutrition, liver disease, overhydration, incr. cap. Permeability, protein-losing states, burns, haemorrhage, general catabolism-sepsis, fever, malignancy, trauma) | ||
*Symptoms – | *Symptoms – edema! (Unresponsive to diuretics or elevation) | ||
=== C-reactive protein === | === C-reactive protein === | ||
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*Also used to detect ectopic pregnancies. | *Also used to detect ectopic pregnancies. | ||
==== Prosate Specific Antigen (PSA) | ==== Prosate Specific Antigen (PSA) ==== | ||
*Monitoring/detecting prostate cancer | *Monitoring/detecting prostate cancer | ||
* | *Maybe raised in benign prostatic hypertrophy | ||
==== CA 125 ==== | ==== CA 125 ==== | ||
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*Elevated in patients with pancreatic tumours | *Elevated in patients with pancreatic tumours | ||
==== CA15-3 | ==== CA15-3 ==== | ||
*Elevated in 70% of patients with metastatic breast cancer. A good marker for monitoring Rx. | *Elevated in 70% of patients with metastatic breast cancer. A good marker for monitoring Rx. | ||
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*Myeloma is a haematological malignancy the symptoms of which are anaemia, bone pain (esp LBP) and pathological #s. | *Myeloma is a haematological malignancy the symptoms of which are anaemia, bone pain (esp LBP) and pathological #s. | ||
== Immunology | == Immunology == | ||
=== Anti-nuclear antibody (ANA) === | === Anti-nuclear antibody (ANA) === | ||
Associated with “connective tissue disease” positive in 95% of SLE, also found in JCA, Sjogren’s syndrome, fibrosing alveolitis | Associated with “connective tissue disease” positive in 95% of SLE, also found in JCA, Sjogren’s syndrome, fibrosing alveolitis | ||
=== Anti-mitochondrial antibodies (AMA) | === Anti-mitochondrial antibodies (AMA) === | ||
positive in >95% of primary biliary cirrhosis. | positive in >95% of primary biliary cirrhosis. | ||
=== Anti-smooth muscle antibodies (ASMA) | === Anti-smooth muscle antibodies (ASMA) === | ||
Positive in 50-70% of autoimmune “lupoid” hepatitis | Positive in 50-70% of autoimmune “lupoid” hepatitis. | ||
=== Rheumatoid factor (RF)<br> === | === Rheumatoid factor (RF)<br> === | ||
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=== Anti-Reticulin Antibodies.<br> === | === Anti-Reticulin Antibodies.<br> === | ||
-Present in Coeliac disease. Also | -Present in Coeliac disease. Also Crohn's and UC. | ||
=== Anti-acetylcholine receptor antibody | === Anti-acetylcholine receptor antibody === | ||
Positive in 80-95% MG | Positive in 80-95% MG | ||
=== Anti-cardiolipin antibody | === Anti-cardiolipin antibody === | ||
Anti-phospholipid syndrome (recurrent abortion, thromboses, thrombocytopaenia) | Anti-phospholipid syndrome (recurrent abortion, thromboses, thrombocytopaenia) | ||
=== Anti-dsDNA antibody | === Anti-dsDNA antibody === | ||
Strongly suggestive of SLE | Strongly suggestive of SLE | ||
=== Anti-ENA (extractable nuclear antigen) Antibody | === Anti-ENA (extractable nuclear antigen) Antibody === | ||
Used to classify connective tissue diseases | Used to classify connective tissue diseases | ||
=== Anti-Intrinsic Factor antibody | === Anti-Intrinsic Factor antibody === | ||
Positive in 70% of pernicious anaemia | Positive in 70% of pernicious anaemia | ||
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=== Anti-neutrophil cytoplasmic antibody (ANCA)<br> === | === Anti-neutrophil cytoplasmic antibody (ANCA)<br> === | ||
Wegener’s granulomatosis, | Wegener’s granulomatosis, microscopic arteritis | ||
=== Anti-thyroid antibody | === Anti-thyroid antibody === | ||
95% positive in Hashimotos thyroiditis<br>90% positive in primary myxoedema<br>18% positive in Graves disease<br> | 95% positive in Hashimotos thyroiditis<br>90% positive in primary myxoedema<br>18% positive in Graves disease<br> | ||
== Haematology | == Haematology == | ||
Coagulation studies -Measure the clotting mechanisms, for diagnosis and extent of disorder. | Coagulation studies -Measure the clotting mechanisms, for diagnosis and extent of disorder. | ||
=== Heparin | === Heparin === | ||
'''Therapeutic administration''' | '''Therapeutic administration''' | ||
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<br> '''International normalized ratio (INR) <br>>10 Life threatening haemorrhage can occur.'''<br>>4.5 Caution re spontaneous bleeding<br>-Always be wary of patients with mild haemorrhage such as haematuria or epistaxis (nosebleed).<br> | <br> '''International normalized ratio (INR) <br>>10 Life threatening haemorrhage can occur.'''<br>>4.5 Caution re spontaneous bleeding<br>-Always be wary of patients with mild haemorrhage such as haematuria or epistaxis (nosebleed).<br> | ||
=== Full Blood Count (FBC) | === Full Blood Count (FBC) === | ||
==== Red blood cell count (RBC) ==== | ==== Red blood cell count (RBC) ==== | ||
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↓anaemia, hyperthyroidism, liver/kidney disease, many CAs (especially haematological) SLE<br>↑haemoconcentration disorders: burns, polycythaemia, COPD, CCF.<br><5 can lead to MI<br>>20 can lead to clogging of capillaries.<br> | ↓anaemia, hyperthyroidism, liver/kidney disease, many CAs (especially haematological) SLE<br>↑haemoconcentration disorders: burns, polycythaemia, COPD, CCF.<br><5 can lead to MI<br>>20 can lead to clogging of capillaries.<br> | ||
==== White blood cell count (WBC) | ==== White blood cell count (WBC) ==== | ||
A useful guide to the severity of a disease process<br>'''Neutrophils 2.5-7.5<br>Lymphocytes 1-3.5<br>Monocytes 0.2-0.8<br>Eosinophils 0.04-0.4<br>Basophils 0.01-0.1''' | A useful guide to the severity of a disease process<br>'''Neutrophils 2.5-7.5<br>Lymphocytes 1-3.5<br>Monocytes 0.2-0.8<br>Eosinophils 0.04-0.4<br>Basophils 0.01-0.1''' | ||
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'''Thrombocytosis'''(↑) malignancies, polycythaemia, RA & other inflammatory diseases, acute infections, <br>'''Thrombocytopaenia'''(↓) toxic affects of drugs, esp. chemotherapy, allergies, anaemias, viral infections, post transfusion, ITP.<br>Caution with low values re spontaneous bleeding, and bruising. | '''Thrombocytosis'''(↑) malignancies, polycythaemia, RA & other inflammatory diseases, acute infections, <br>'''Thrombocytopaenia'''(↓) toxic affects of drugs, esp. chemotherapy, allergies, anaemias, viral infections, post transfusion, ITP.<br>Caution with low values re spontaneous bleeding, and bruising. | ||
<div class="researchbox"> | <div class="researchbox"> | ||
== References == | == References == | ||
Revision as of 07:06, 6 December 2017
Original Editor - John Mitchell
Top Contributors - Rachael Lowe, Scott Buxton, Lucinda hampton, Admin, Jin Yoo, Kim Jackson, Mason Trauger, Evan Thomas, Naomi O'Reilly, Mariam Hashem, WikiSysop and Adam Vallely Farrell
An Introduction To Pathology[edit | edit source]
Different clinical areas which can be tested:
- Pathology
- Biochemistry
- Haematology (blood bank)
- Microbiology
- Histology
- Cytology
- Immunology
Use of Pathology Tests[edit | edit source]
Differential Diagnosis: to confirm or refute
Prognosis: risk factors
Monitoring: progress & treatment
Screening: subclinical presence of pathology
Interpretation[edit | edit source]
Is it normal?
Is it different?
Is it consistent with clinical findings?
Factors affecting results[edit | edit source]
- Age and stage of development
- Ethnicity
- Sex
- Pregnancy
- Posture
- Exercise
- Stress
- Nutritional state
- Time
- Other medical intervention
Chemical Pathology[edit | edit source]
NB reference ranges apply to adults only and are laboratory-specific and not applicable to every lab result
Water and sodium[edit | edit source]
Na: 135-145 mmol/l
↓Na - Hyponatremia (from skin, kidneys, gut) – weakness, postural hypotension, syncope, wt loss, cv changes, decreased skin turgor.
↑Na - Hypernatremia (incr. intake, decr. Excretion) – oedema, pulmonary oedema, HT, effusions
Potassium[edit | edit source]
K: 3.4-5.2 mmol/l
↓K- Hypokalemia (alkalosis, RF, D&V,diuretics) – Weakness, ileus, hypotonia, confusion, depression, arrhythmias, alkalosis.
↑K- Hyperkalemia (catabolism, acidosis, RF) – Cardiac arrest with VF.
Renal Function Tests - Urea & creatinine[edit | edit source]
U: 2.5-6.5 mmol/l Cr: 60-120μmol/l
Both should rise together in renal failure. Creat is the more accurate measurement, urea is affected more by diet and dehydration.
Creatinine Clearance Crcl: 100-120mls/min
Measures glomerular filtration rate and permeability
Enzymes[edit | edit source]
- Non-specific
- Have a specific time window of elevation
- Temperature specific
Alkaline phosphatase[edit | edit source]
alk: 30-120 IU/l
- ↑ - Pagets, osteomalacia, bone tumours, healing #, osteomyelitis.
Cholestasis, cirrhosis, hepatitis, liver tumour.
Alanine transaminase[edit | edit source]
Alt/GPT: <40 IU/l
- ↑ - Acute and chronic hepatitis, liver necrosis, tissue hypoxaemia and crush injuries, cholestasis & other liver diseases.
Creatine kinase CK: ♂40-215 ♀40-185 IU/l
- ↑ - MI, rhabdomyolisis, Sk. Muscle trauma, MD, severe exercise
Enzymes and MIs
- -Troponin T (Trop T) rises within hours & remains elevated for days. Results reported as neg, pos or some myocardial damage.
-CK:
- At 3hrs, 25% of MIs have raised CKs
- At 6hrs, 72% “ “ “ “ “
- At 9hrs, 97% “ “ “ “ “
Gamma glutamyl tranferase[edit | edit source]
γGT/GTP: ♂<65 ♀<55 IU/l
- ↑ Liver disease esp. Cholestasis, alcoholic liver disease, cirrhosis, hepatitis
Amylase[edit | edit source]
Amy: <100 IU/l
- ↑ Acute pancreatitis
other abdo disorders, RF
Thyroid diseases
[edit | edit source]
Hyperthyroidism (Graves disease, multinodular goiter, adenoma) – weight loss, sweating, palpitation, angina, tremor, diarrhea, muscle weakness, goiter, eyelid retraction.
Hypothyroidism (Hashimotos disease, post surgery, congenital, secondary to pituitary/hypothalamic disease)
- lethargy, cold intolerance, dry coarse skin & hair, hoarseness, wt gain, slow reflexes & muscle relaxation. Others including: anaemia, psychosis, constipation bradycardia, CTS, infertility.
Thyroid Function Testing:
TSH 0.3-5mU/l
>15mU/l - 1° hypothyroidism
0.3-5mU/l - euthyroid (normal)
<0.3mU/l - Further investigations!
Interpretation complicated by:
-Many medications, hormones
-Any acute illness – “sick euthyroidism” all thyroid tests are low.
-Recovery – TSH raised.
Glucose:[edit | edit source]
Fasting 3-5.5 mmol/l
>7.8 – diagnostic of DM
5.5-7.8 – impaired glucose tolerance
Hyperglycaemia
Thirst, polyuria, dehydration, hypotension, tachycardia, drowsiness.
(and for IDDM, DKA) ketosis, hyperventilation, vomiting.
Hypoglycaemia
Tiredness, confusion, detachment, ataxia, blurred vision, dizziness, paraesthesia, hemiparesis, convulsions, coma
Lactate[edit | edit source]
<2.0 mmol/l
↑ mainly due to tissue hypoxia (decreased perfusion or decr. PO2)
Albumin[edit | edit source]
alb: 36-50 g/l
- Maintains oncotic pressure (keeps fluid in vessels)
- Transports small drugs, calcium & hormones
- ↓ (Many causes incl. malnutrition, liver disease, overhydration, incr. cap. Permeability, protein-losing states, burns, haemorrhage, general catabolism-sepsis, fever, malignancy, trauma)
- Symptoms – edema! (Unresponsive to diuretics or elevation)
C-reactive protein[edit | edit source]
CRP: <5mg/l
An ‘acute phase’ protein
-Monitoring infections (>100, more likely to be bacterial)
-Distinguishing between AI diseases and active infection
-Monitoring RA Rx
-Checking for post-op infection
More sensitive than ESR
Uric acid[edit | edit source]
0.1-0.4 mmol/l
Incr. production/decr. Excretion → gout (exquisite pain and inflammation often in 1st MTP joint)
>0.54 mM → 50% chance of developing gout
Tumour Markers
[edit | edit source]
- Chemicals related to the presence/progress of a tumour
- Either secreted by tumours or cell surface antigens
- Of greater prognostic than diagnostic use, should always be interpreted in the light of clinical and other diagnostic findings.
Carcinoembryonic Antigen[edit | edit source]
CEA 2-5μg/l
- Monitoring/detection of colorectal, gastric, breast, bronchial, bronchial and some ovarian cancers.
- Modestly elevated levels in a variety of non-malignancies
Alpha Fetoprotein[edit | edit source]
AFP <9 KU/l
- Monitoring/detecting liver cancers, testicular cancer.
- Also raised in pregnancy, hepatic regeneration.
Human Chorionic Gonadotrophin[edit | edit source]
βHCG <5IU/l
- Diagnosis and monitoring of choriocarcinoma, also testicular tumours.
- Also used to detect ectopic pregnancies.
Prosate Specific Antigen (PSA)[edit | edit source]
- Monitoring/detecting prostate cancer
- Maybe raised in benign prostatic hypertrophy
CA 125[edit | edit source]
<35 IU/l
- 96% of patients with ovarian cancer have raised levels
CA 19-9[edit | edit source]
<60 IU/l
- Elevated in patients with pancreatic tumours
CA15-3[edit | edit source]
- Elevated in 70% of patients with metastatic breast cancer. A good marker for monitoring Rx.
Paraproteins
[edit | edit source]
- Elevated in 98% of patients with myeloma (and other malignancies of B-cells.
- Myeloma is a haematological malignancy the symptoms of which are anaemia, bone pain (esp LBP) and pathological #s.
Immunology[edit | edit source]
Anti-nuclear antibody (ANA)[edit | edit source]
Associated with “connective tissue disease” positive in 95% of SLE, also found in JCA, Sjogren’s syndrome, fibrosing alveolitis
Anti-mitochondrial antibodies (AMA)[edit | edit source]
positive in >95% of primary biliary cirrhosis.
Anti-smooth muscle antibodies (ASMA)[edit | edit source]
Positive in 50-70% of autoimmune “lupoid” hepatitis.
Rheumatoid factor (RF)
[edit | edit source]
(anti-IgG antibodies) Positive in 70% of RA (but lots of false positives). Should only be used to screen, NOT monitor, (use C-RP instead)
Anti-Reticulin Antibodies.
[edit | edit source]
-Present in Coeliac disease. Also Crohn's and UC.
Anti-acetylcholine receptor antibody[edit | edit source]
Positive in 80-95% MG
Anti-cardiolipin antibody[edit | edit source]
Anti-phospholipid syndrome (recurrent abortion, thromboses, thrombocytopaenia)
Anti-dsDNA antibody[edit | edit source]
Strongly suggestive of SLE
Anti-ENA (extractable nuclear antigen) Antibody[edit | edit source]
Used to classify connective tissue diseases
Anti-Intrinsic Factor antibody[edit | edit source]
Positive in 70% of pernicious anaemia
Anti-neutrophil cytoplasmic antibody (ANCA)
[edit | edit source]
Wegener’s granulomatosis, microscopic arteritis
Anti-thyroid antibody[edit | edit source]
95% positive in Hashimotos thyroiditis
90% positive in primary myxoedema
18% positive in Graves disease
Haematology[edit | edit source]
Coagulation studies -Measure the clotting mechanisms, for diagnosis and extent of disorder.
Heparin[edit | edit source]
Therapeutic administration
- DVT, PE
- MI, Unstable Angina
Prophylactic administration
- >30 mins GA with post-op bed rest.
- High risk patients.
Pregnancy - Relevant side effects
- Haemorrhage
- Thrombocytopaenia
Monitoring of Heparin
Low dose subcut. –
no laboratory control required
Continuous iv infusion or full dose subcut. –
APTT (activated partial thromboplastin time) should
be between 50-75 seconds. Caution with high values
(>100) re spontaneous bleeding.
Warfarin
[edit | edit source]
- Patients require close monitoring of INR (International Normalized Ratio).
- Target INR ranges are 2-3 for moderate anticoagulation and 3-4.5 for more intensive therapy.
- These doses may fluctuate with no clinical significance.
Warfarin requirements may be dramatically changed by:
- Illness
- Change in diet
- Change in other medication
International normalized ratio (INR)
>10 Life threatening haemorrhage can occur.
>4.5 Caution re spontaneous bleeding
-Always be wary of patients with mild haemorrhage such as haematuria or epistaxis (nosebleed).
Full Blood Count (FBC)[edit | edit source]
Red blood cell count (RBC)[edit | edit source]
♂4.5-6.5 ♀3.5-5.8
↓anaemias, Hodgkins disease, myeloma, leukaemia, haemorrhage, SLE, rheumatic fever and chronic infection.
↑polycythaemia, renal disorders, decr. plasma vol: (severe burns, shock, vomiting)
Haemoglobin (Hb)[edit | edit source]
♂13-18 ♀12-16
↓anaemia, hyperthyroidism, liver/kidney disease, many CAs (especially haematological) SLE
↑haemoconcentration disorders: burns, polycythaemia, COPD, CCF.
<5 can lead to MI
>20 can lead to clogging of capillaries.
White blood cell count (WBC)[edit | edit source]
A useful guide to the severity of a disease process
Neutrophils 2.5-7.5
Lymphocytes 1-3.5
Monocytes 0.2-0.8
Eosinophils 0.04-0.4
Basophils 0.01-0.1
Neutrophilia(↑) bacterial infections, gout, uraemia, poisoning, haemorrhage, haemolysis, necrosis.
Neutropaenia(↓) bacterial infections (poor prognosis) viral infections, certain anaemias, blood cancers, anaphylactic shock.
Eosinophilia(↑) Allergic reactions, parasitic diseases, certain blood cancers, skin infections, immunodeficiency disorders.
-Other variations occur in the specific white blood cell differential count with conditions such as: anaemias, blood cancers, infections, and certain inflammatory disorders.
Platelets (150-400)[edit | edit source]
Thrombocytosis(↑) malignancies, polycythaemia, RA & other inflammatory diseases, acute infections,
Thrombocytopaenia(↓) toxic affects of drugs, esp. chemotherapy, allergies, anaemias, viral infections, post transfusion, ITP.
Caution with low values re spontaneous bleeding, and bruising.